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PDHA2 Polyclonal antibody

PDHA2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17134-1-AP

Print datasheet

Synonyms

PDHA2, PDHAL, PDHE1 A type II

验证数据展示

  • WB analysis using 17134-1-AP

    WB analysis using 17134-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17134-1-AP (PDHA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 17134-1-AP

    IHC staining of mouse testis using 17134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17134-1-AP (PDHA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, human placenta tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17134-1-AP is PDHA2 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PDHA2 fusion protein Ag10882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 2
Synonyms PDHA2, PDHAL, PDHE1 A type II
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC030697
Gene Symbol PDHA2
Gene ID (NCBI) 5161
RRIDAB_2878352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29803
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDHA2 antibody 17134-1-APDownload protocol
IHC protocol for PDHA2 antibody 17134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols