- Featured Product
- KD/KO Validated
PDH E1 Alpha Monoclonal antibody
PDH E1 Alpha Monoclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2B3C10
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HEK293 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human liver cancer tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 66119-1-Ig is PDH E1 Alpha Fusion Protein expressed in E. coli.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | PDH E1 Alpha fusion protein Ag12556 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
Synonyms | PDH E1 Alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC002406 |
Gene Symbol | PDHA1 |
Gene ID (NCBI) | 5160 |
RRID | AB_2881518 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08559 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
IHC protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
IF protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
IP protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
J Cell Biol PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy. | ||
Cell Rep A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria. | ||
Toxicol Appl Pharmacol Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis. | ||
Immunobiology The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus. | ||
Nat Metab MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion.
|