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验证数据展示

  • WB analysis using 66119-1-Ig (same clone as 66119-1-PBS)

    WB analysis using 66119-1-Ig (same clone as 66119-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

    WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

    WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 66119-1-Ig (same clone as 66119-1-PBS)

    IP experiment of K-562 using 66119-1-Ig (same clone as 66119-1-PBS)

    IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 4ug; Detection:66119-1-Ig 1:10000) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDHA1 antibody included
  • IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDHA1 antibody included
  • IF Staining of HeLa using 66119-1-Ig (same clone as 66119-1-PBS)

    IF Staining of HeLa using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDH E1 Alpha antibody (66119-1-Ig, Clone: 2B3C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), PDH E1 Alpha antibody (18068-1-AP, red). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PDH E1 Alpha Monoclonal antibody, PBS Only

PDH E1 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66119-1-PBS

产品说明书

CloneNo. 2B3C10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

PDH E1α, PDHA1, 2B3C10, EC:1.2.4.1, PDHA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

产品信息

66119-1-PBS targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12556

Product name: Recombinant human PDHA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 41-390 aa of BC002406

Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名 PDH E1α, PDHA1, 2B3C10, EC:1.2.4.1, PDHA
计算分子量 43 kDa
观测分子量 39-48 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
RRIDAB_2881518
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08559
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

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