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CoraLite®594-conjugated PDH E1 Alpha Monoclonal antibody

PDH E1 Alpha Monoclonal Antibody for WB, FC (Intra)
Cat No. CL594-66119

产品说明书

CloneNo. 2B3C10

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra)

PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66119-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66119 targets PDH E1 Alpha in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag12556

Product name: Recombinant human PDHA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 41-390 aa of BC002406

Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名 PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
RRIDAB_2919949
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08559
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

实验方案

Product Specific Protocols
WB protocol for CL594 PDH E1 Alpha antibody CL594-66119Download protocol
FC protocol for CL594 PDH E1 Alpha antibody CL594-66119Download protocol
Standard Protocols
Click here to view our Standard Protocols
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