验证数据展示
经过测试的应用
| Positive FC detected in | NIH/3T3 cells |
For other applications, we recommend the unconjugated version of this antibody, 98256-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98256 targets PDGFR alpha/CD140a in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | PDGFR alpha/CD140a fusion protein Eg1625 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | platelet derived growth factor receptor, alpha polypeptide |
| 别名 | 242025B3, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a |
| 计算分子量 | 123kDa |
| GenBank蛋白编号 | NM_001083316.2 |
| 基因名称 | Pdgfra |
| Gene ID (NCBI) | 18595 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P26618-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 PDGFR alpha/CD140a antibody CL647-98256 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |