×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 27406-1-AP (same clone as 27406-1-PBS)

    WB analysis using 27406-1-AP (same clone as 27406-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

    IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

    IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 27406-1-AP (same clone as 27406-1-PBS)

    IF Staining of NIH/3T3 using 27406-1-AP (same clone as 27406-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PDCD1LG2/PD-L2 antibody (27406-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

PD-L2/CD273 Polyclonal antibody, PBS Only

PD-L2/CD273 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27406-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

27406-1-PBS targets PD-L2/CD273 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26384

Product name: Recombinant human PDCD1LG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-220 aa of BC074766

Sequence: LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 programmed cell death 1 ligand 2
别名 PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC
计算分子量 273 aa, 31 kDa
观测分子量 45 kDa
GenBank蛋白编号BC074766
基因名称 PD-L2
Gene ID (NCBI) 80380
RRIDAB_3085954
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQ51
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).

||
New chat

Able™

正在加载，请稍候...