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PDCD1LG2/PD-L2 Polyclonal antibody

PDCD1LG2/PD-L2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 27406-1-AP

Print datasheet

Synonyms

B7 DC, B7DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PD L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, Programmed death ligand 2

验证数据展示

  • WB analysis using 27406-1-AP

    WB analysis using 27406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, Raji cells, mouse heart tissue, rat heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27406-1-AP targets PDCD1LG2/PD-L2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PDCD1LG2/PD-L2 fusion protein Ag26384 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name programmed cell death 1 ligand 2
Synonyms B7 DC, B7DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PD L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, Programmed death ligand 2
Calculated Molecular Weight 273 aa, 31 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC074766
Gene Symbol PDCD1LG2
Gene ID (NCBI) 80380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ51
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).

实验方案

Product Specific Protocols
WB protocol for PDCD1LG2/PD-L2 antibody 27406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols