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验证数据展示

  • WB analysis using 27406-1-AP

    WB analysis using 27406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27406-1-AP

    IHC staining of mouse heart using 27406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 27406-1-AP

    IHC staining of mouse heart using 27406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 27406-1-AP

    IF Staining of NIH/3T3 using 27406-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PDCD1LG2/PD-L2 antibody (27406-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PD-L2/CD273 Polyclonal antibody

PD-L2/CD273 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27406-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, Raji cells, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27406-1-AP targets PD-L2/CD273 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26384

Product name: Recombinant human PDCD1LG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-220 aa of BC074766

Sequence: LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 programmed cell death 1 ligand 2
别名 PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC
计算分子量 273 aa, 31 kDa
观测分子量 45 kDa
GenBank蛋白编号BC074766
基因名称 PD-L2
Gene ID (NCBI) 80380
RRIDAB_3085954
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQ51
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).

实验方案

Product Specific Protocols
IF protocol for PD-L2/CD273 antibody 27406-1-APDownload protocol
WB protocol for PD-L2/CD273 antibody 27406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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