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验证数据展示

  • WB analysis using 86075-1-RR (same clone as 86075-1-PBS)

    WB analysis using 86075-1-RR (same clone as 86075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86075-1-RR (PCNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 86075-1-RR (same clone as 86075-1-PBS)

    IHC staining of human colon using 86075-1-RR (same clone as 86075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86075-1-RR (PCNP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86075-1-RR (same clone as 86075-1-PBS)

    IF Staining of HeLa using 86075-1-RR (same clone as 86075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (86075-1-RR, Clone: 250576B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86075-1-RR

    Affinity and Kinetic Characterization of 86075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86075-1-RR against Human PCNP were performed. The affinity constant is 1.41 nM.

PCNP Recombinant monoclonal antibody, PBS Only

PCNP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86075-1-PBS

产品说明书

CloneNo. 250576B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86075-1-PBS targets PCNP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30951

Product name: Recombinant human PCNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of BC013916

Sequence: MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PEST proteolytic signal containing nuclear protein
别名 PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
计算分子量 19 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC013916
基因名称 PCNP
Gene ID (NCBI) 57092
RRIDAB_3744602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WW12
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PEST-containing nuclear protein (PCNP), characterized by a PEST motif rich in proline (P), glutamic acid (E), serine (S), and threonine (T), is firstly identified by database mining. The PCNP protein with the PEST motif is highly subjected to ubiquitination. PCNP may act as a transcriptional factor, cell cycle regulatory protein and tumor regulating nuclear protein.(PMID: 35813472, PMID: 32548978, PMID: 31487123)

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