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验证数据展示

  • WB analysis using 86075-1-RR

    WB analysis using 86075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86075-1-RR (PCNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 86075-1-RR

    IHC staining of human colon using 86075-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86075-1-RR (PCNP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 86075-1-RR

    IF Staining of HeLa using 86075-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (86075-1-RR, Clone: 250576B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86075-1-RR

    Affinity and Kinetic Characterization of 86075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86075-1-RR against Human PCNP were performed. The affinity constant is 1.41 nM.

PCNP Recombinant antibody

PCNP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86075-1-RR

产品说明书

CloneNo. 250576B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells, Raji cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86075-1-RR targets PCNP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PCNP fusion protein Ag30951 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PEST proteolytic signal containing nuclear protein
别名 PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
计算分子量 19 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC013916
基因名称 PCNP
Gene ID (NCBI) 57092
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WW12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCNP antibody 86075-1-RRDownload protocol
IF protocol for PCNP antibody 86075-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols