×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86075-1-RR

    WB analysis using 86075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86075-1-RR (PCNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 86075-1-RR

    IHC staining of human colon using 86075-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86075-1-RR (PCNP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 86075-1-RR

    IF Staining of HeLa using 86075-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (86075-1-RR, Clone: 250576B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86075-1-RR

    Affinity and Kinetic Characterization of 86075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86075-1-RR against Human PCNP were performed. The affinity constant is 1.41 nM.

PCNP Recombinant monoclonal antibody

PCNP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86075-1-RR

产品说明书

CloneNo. 250576B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86075-1-RR targets PCNP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30951

Product name: Recombinant human PCNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of BC013916

Sequence: MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PEST proteolytic signal containing nuclear protein
别名 PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
计算分子量 19 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC013916
基因名称 PCNP
Gene ID (NCBI) 57092
RRIDAB_3744602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WW12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEST-containing nuclear protein (PCNP), characterized by a PEST motif rich in proline (P), glutamic acid (E), serine (S), and threonine (T), is firstly identified by database mining. The PCNP protein with the PEST motif is highly subjected to ubiquitination. PCNP may act as a transcriptional factor, cell cycle regulatory protein and tumor regulating nuclear protein.(PMID: 35813472, PMID: 32548978, PMID: 31487123)

实验方案

Product Specific Protocols
IF protocol for PCNP antibody 86075-1-RRDownload protocol
IHC protocol for PCNP antibody 86075-1-RRDownload protocol
WB protocol for PCNP antibody 86075-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...