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验证数据展示

  • IHC staining of mouse heart using 84979-2-RR

    IHC staining of mouse heart using 84979-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84979-2-RR (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCM1 antibody included
  • FC experiment of K562 using 84979-2-RR

    FC experiment of K562 using 84979-2-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-2-RR, Clone:242199D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of HepG2 using 84979-2-RR

    IF Staining of HepG2 using 84979-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCM1 antibody (84979-2-RR, Clone: 242199D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84979-2-RR

    IF Staining of HeLa using 84979-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCM1 antibody (84979-2-RR, Clone: 242199D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84979-2-RR

    Affinity and Kinetic Characterization of 84979-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84979-2-RR against Human PCM1 were performed. The affinity constant is 3.24 nM.

PCM1 Recombinant monoclonal antibody

PCM1 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84979-2-RR

产品说明书

CloneNo. 242199D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

242199D6, hPCM 1, hPCM-1, PCM 1, PCM-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inK562
Planning an IHC experiment? We recommend our IHCeasy PCM1 Ready-To-Use IHC Kit. PCM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84979-2-RR targets PCM1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13977

Product name: Recombinant human PCM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-254 aa of BC000453

Sequence: MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pericentriolar material 1
别名 242199D6, hPCM 1, hPCM-1, PCM 1, PCM-1
计算分子量 2024 aa, 228 kDa
GenBank蛋白编号BC000453
基因名称 PCM1
Gene ID (NCBI) 5108
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ15154
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

实验方案

Product Specific Protocols
IF protocol for PCM1 antibody 84979-2-RRDownload protocol
IHC protocol for PCM1 antibody 84979-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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