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验证数据展示

  • IF Staining of MCF-7 using 14892-1-AP (same clone as 14892-1-PBS)

    IF Staining of MCF-7 using 14892-1-AP (same clone as 14892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK1/2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 14892-1-AP

    WB analysis of mouse brain using 14892-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14892-1-AP

    IHC staining of human breast cancer using 14892-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK2 antibody included
  • IHC staining of human breast cancer using 14892-1-AP

    IHC staining of human breast cancer using 14892-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK2 antibody included
  • FC experiment of HepG2 using 14892-1-AP (same clone as 14892-1-PBS)

    FC experiment of HepG2 using 14892-1-AP (same clone as 14892-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PCK1/2 Polyclonal antibody (14892-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 14892-1-AP (same clone as 14892-1-PBS)

    WB analysis of mouse liver using 14892-1-AP (same clone as 14892-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

    IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK2 antibody included

PCK1/2 Polyclonal antibody, PBS Only

PCK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 14892-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PCK2, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.

产品信息

14892-1-PBS targets PCK1/2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6681

Product name: Recombinant human PCK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-640 aa of BC001454

Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 2 (mitochondrial)
别名 PCK2, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M
计算分子量 71 kDa
观测分子量 66-71 kDa
GenBank蛋白编号BC001454
基因名称 PCK2
Gene ID (NCBI) 5106
RRIDAB_2160044
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16822
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.

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