PCK1/2 Polyclonal antibody

PCK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14892-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

PCK2, Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue, mouse kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14892-1-AP targets PCK1/2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 PCK1/2 fusion protein Ag6681 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 2 (mitochondrial)
别名 PCK2, Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2
计算分子量 71 kDa
观测分子量 66-71 kDa
GenBank蛋白编号BC001454
基因名称 PCK2
Gene ID (NCBI) 5106
RRIDAB_2160044
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16822
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.

实验方案

Product Specific Protocols
WB protocol for PCK1/2 antibody 14892-1-APDownload protocol
IHC protocol for PCK1/2 antibody 14892-1-APDownload protocol
IF protocol for PCK1/2 antibody 14892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.

Authors - Fengqin Dong
mouseWB

Mol Nutr Food Res

Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice

Authors - Songfeng Yu
mouseWB

J Nat Prod

Effect of 1-Deoxynojirimycin Isolated from Mulberry Leaves on Glucose Metabolism and Gut Microbiota in a Streptozotocin-Induced Diabetic Mouse Model

Authors - Teng-Gen Hu
ratIF

Mol Neurobiol

Phosphoenolpyruvate Carboxykinase (PCK) in the Brain Gluconeogenic Pathway Contributes to Oxidative and Lactic Injury After Stroke.

Authors - Xiaokun Geng
ratWB

Front Pharmacol

Bicyclol Regulates Hepatic Gluconeogenesis in Rats with Type 2 Diabetes and Non-alcoholic Fatty Liver Disease by Inhibiting Inflammation.

Authors - Hongxue Li
mouseWB

Food Funct

Polysaccharides from Armillariella tabescens mycelia ameliorate insulin resistance in type 2 diabetic mice.

Authors - Rui Yang
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