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CoraLite®555-conjugated PCK1 Monoclonal antibody

PCK1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, human

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2F11B5

Cat No : CL555-66862

Print datasheet

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

验证数据展示

  • IF Staining of HeLa using CL555-66862

    IF Staining of HeLa using CL555-66862

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 PCK1 antibody (CL555-66862, Clone: 2F11B5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66862-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66862 targets PCK1 in IF applications and shows reactivity with mouse, rat, pig, human samples.

Tested Applications IF/ICC
Tested Reactivity mouse, rat, pig, human
Immunogen PCK1 fusion protein Ag9720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Synonyms PCK1, PEPCK C, PEPCK1, PEPCKC
Calculated Molecular Weight 622 aa, 69 kDa
Observed Molecular Weight 65-69 kDa
GenBank Accession NumberBC023978
Gene Symbol PCK1
Gene ID (NCBI) 5105
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35558
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

实验方案

Product Specific Protocols
IF protocol for CL555 PCK1 antibody CL555-66862Download protocol
Standard Protocols
Click here to view our Standard Protocols