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验证数据展示

  • IF Staining of HepG2 using CL488-16754

    IF Staining of HepG2 using CL488-16754

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PCK1/2 antibody (CL488-16754) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PCK1/2 Polyclonal antibody

PCK1/2 Polyclonal Antibody for IF/ICC
Cat No. CL488-16754

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

PCK1, Serine-protein kinase PCK1, PEPCK-C, PEPCK1, EC:4.1.1.32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16754-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16754 targets PCK1/2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 PCK1/2 fusion protein Ag10261 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 1 (soluble)
别名 PCK1, Serine-protein kinase PCK1, PEPCK-C, PEPCK1, EC:4.1.1.32
计算分子量 622 aa, 69 kDa
观测分子量 66-69 kDa
GenBank蛋白编号BC023978
基因名称 PCK1
Gene ID (NCBI) 5105
RRIDAB_3083990
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35558
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). This antibody has weak cross-reactivity with PCK2 protein due to high sequence homology between PCK1 and PCK2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PCK1/2 antibody CL488-16754Download protocol
Standard Protocols
Click here to view our Standard Protocols