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验证数据展示

  • WB analysis using 85408-5-RR (same clone as 85408-5-PBS)

    WB analysis using 85408-5-RR (same clone as 85408-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85408-5-RR (PARN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

  • IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR (same clone as 85408-5-PBS)

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR (same clone as 85408-5-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARN antibody included
  • IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR (same clone as 85408-5-PBS)

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR (same clone as 85408-5-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARN antibody included
  • FC experiment of A431 using 85408-5-RR (same clone as 85408-5-PBS)

    FC experiment of A431 using 85408-5-RR (same clone as 85408-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PARN Recombinant monoclonal antibody (85408-5-RR, Clone:242993F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85408-5-RR

    Affinity and Kinetic Characterization of 85408-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85408-5-RR against Human PARN were performed. The affinity constant is 96.9 pM.

PARN Recombinant monoclonal antibody, PBS Only

PARN Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 85408-5-PBS

产品说明书

CloneNo. 242993F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

242993F5, DAN, EC:3.1.13.4, Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PARN Ready-To-Use IHC Kit. PARN primary antibody included.

产品信息

85408-5-PBS targets PARN in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4774

Product name: Recombinant human PARN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC050029

Sequence: MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poly(A)-specific ribonuclease (deadenylation nuclease)
别名 242993F5, DAN, EC:3.1.13.4, Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease
计算分子量 639 aa, 73 kDa
观测分子量 74 kDa
GenBank蛋白编号BC050029
基因名称 PARN
Gene ID (NCBI) 5073
RRIDAB_3744201
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95453
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly(A)-specific ribonuclease (PARN) is a deadenylase enzyme that is present in mammalian cells. As a deadenylase, PARN interacts with the cap and the poly(A) tail of mRNA to control the length of the poly(A) tail and regulate gene expression. Therefore, it plays a role in mRNA degradation in the nucleus and cytoplasm.

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