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验证数据展示

  • WB analysis using 85408-5-RR

    WB analysis using 85408-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85408-5-RR (PARN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARN antibody included
  • IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARN antibody included
  • Affinity and Kinetic Characterization of 85408-5-RR

    Affinity and Kinetic Characterization of 85408-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85408-5-RR against Human PARN were performed. The affinity constant is 96.9 pM.

PARN Recombinant monoclonal antibody

PARN Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85408-5-RR

产品说明书

CloneNo. 242993F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242993F5, DAN, EC:3.1.13.4, Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MDA-MB-453 cells, HeLa cells, HEK-293T cells, HepG2 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inHuman Breast Cancer(Her2+;ER-;PR-)
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PARN Ready-To-Use IHC Kit. PARN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85408-5-RR targets PARN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4774

Product name: Recombinant human PARN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC050029

Sequence: MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poly(A)-specific ribonuclease (deadenylation nuclease)
别名 242993F5, DAN, EC:3.1.13.4, Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease
计算分子量 639 aa, 73 kDa
观测分子量 74 kDa
GenBank蛋白编号BC050029
基因名称 PARN
Gene ID (NCBI) 5073
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95453
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Poly(A)-specific ribonuclease (PARN) is a deadenylase enzyme that is present in mammalian cells. As a deadenylase, PARN interacts with the cap and the poly(A) tail of mRNA to control the length of the poly(A) tail and regulate gene expression. Therefore, it plays a role in mRNA degradation in the nucleus and cytoplasm.

实验方案

Product Specific Protocols
IHC protocol for PARN antibody 85408-5-RRDownload protocol
WB protocol for PARN antibody 85408-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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