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验证数据展示

  • IF Staining of HeLa using CL488-68042

    IF Staining of HeLa using CL488-68042

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PAIP1 antibody (CL488-68042, Clone: 2H9D4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68042

    FC experiment of HeLa using CL488-68042

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PAIP1 (CL488-68042, Clone:2H9D4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68042

产品说明书

CloneNo. 2H9D4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rabbit

应用

IF/ICC, FC (Intra)

别名

2H9D4, PABP interacting protein 1, PABP-interacting protein 1, PAIP 1, PAIP-1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68042-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68042 targets PAIP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rabbit
免疫原

CatNo: Ag16773

Product name: Recombinant human PAIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 102-400 aa of BC005295

Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 poly(A) binding protein interacting protein 1
别名 2H9D4, PABP interacting protein 1, PABP-interacting protein 1, PAIP 1, PAIP-1
计算分子量 70 kDa
观测分子量40-45kDa, 62kDa
GenBank蛋白编号BC005295
基因名称 PAIP1
Gene ID (NCBI) 10605
RRIDAB_2923867
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H074
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PAIP1 antibody CL488-68042Download protocol
IF protocol for CL Plus 488 PAIP1 antibody CL488-68042Download protocol
Standard Protocols
Click here to view our Standard Protocols
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