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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PADI4 Polyclonal antibody

PADI4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-17373

Print datasheet

Synonyms

HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV

验证数据展示

  • IF Staining of A549 using CL488-17373

    IF Staining of A549 using CL488-17373

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 PADI4 antibody (CL488-17373) at dilution of 1:200.

  • FC experiment of A549 using CL488-17373

    FC experiment of A549 using CL488-17373

    1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PADI4 (CL488-17373) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inA549 cells
Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 17373-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17373 targets PADI4 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen PADI4 fusion protein Ag11068 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidyl arginine deiminase, type IV
Synonyms HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV
Calculated Molecular Weight 663 aa, 74 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC025718
Gene Symbol PADI4
Gene ID (NCBI) 23569
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM07
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PADI4 antibody CL488-17373Download protocol
FC protocol for CL Plus 488 PADI4 antibody CL488-17373Download protocol
Standard Protocols
Click here to view our Standard Protocols