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KD/KO Validated

PADI4 Polyclonal antibody

PADI4 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat No : 17373-1-AP

Print datasheet

Synonyms

HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV

验证数据展示

IF Staining of A549 using 17373-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 17373-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PADI4 (17373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of Purified protein using 17373-1-AP
WB result of PADI4 antibody (17373-1-AP) with purified protein by T.J. Hollingsworth, Ph.D., University of Tennessee Health Sciences Center.
WB analysis using 17373-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 17373-1-AP
IP result of anti-PADI4 (IP:17373-1-AP, 4ug; Detection:17373-1-AP 1:500) with mouse spleen tissue lysate 4800ug.
IHC staining of human breast cancer using 17373-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 17373-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 17373-1-AP (PADI4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human breast cancer using 17373-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 17373-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, Purified protein, HepG2 cells, C6 cells, RAW 264.7 cells
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A549 cells
Positive FC detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:200-1:800
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 17 publications below
IHC	See 6 publications below
IF	See 12 publications below
IP	See 2 publications below
CoIP	See 1 publications below

产品信息

17373-1-AP targets PADI4 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IP, IF, IHC, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	PADI4 fusion protein Ag11068 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	peptidyl arginine deiminase, type IV
Synonyms	HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV
Calculated Molecular Weight	663 aa, 74 kDa
Observed Molecular Weight	67 kDa
GenBank Accession Number	BC025718
Gene Symbol	PADI4
Gene ID (NCBI)	23569
RRID	AB_2878398
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UM07
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)

实验方案

Product Specific Protocols
WB protocol for PADI4 antibody 17373-1-AP	Download protocol
IHC protocol for PADI4 antibody 17373-1-AP	Download protocol
IF protocol for PADI4 antibody 17373-1-AP	Download protocol
IP protocol for PADI4 antibody 17373-1-AP	Download protocol
FC protocol for PADI4 antibody 17373-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
rat	WB	Int J Mol Sci Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis. Authors - Szilvia Török View Article
mouse	WB	RMD Open IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis. Authors - Ayano Yahagi View Article
human	WB, IF	Transl Stroke Res Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage. Authors - Hanhai Zeng View Article
mouse	WB	Virulence Extracellular trap can be trained as a memory response. Authors - Yu Gao View Article
mouse	IF	FASEB J Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background. Authors - Kiyomi Mizugishi View Article
mouse	WB	Front Immunol TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage. Authors - Xinyan Wu View Article

PADI4 Polyclonal antibody

PADI4 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

IF Staining of A549 using 17373-1-AP

FC experiment of HeLa using 17373-1-AP

WB analysis of Purified protein using 17373-1-AP

WB analysis using 17373-1-AP

IP experiment of mouse spleen using 17373-1-AP

IHC staining of human breast cancer using 17373-1-AP

IF Staining of A549 using 17373-1-AP

IHC staining of human breast cancer using 17373-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.

IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.

Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.

Extracellular trap can be trained as a memory response.

Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.

TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.

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