验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, mouse spleen tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17373-1-AP targets PADI4/PAD4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11068 Product name: Recombinant human PADI4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 317-663 aa of BC025718 Sequence: EDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRGKEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peptidyl arginine deiminase, type IV |
| 别名 | PADI4, EC:3.5.3.15, HL 60 PAD, HL-60 PAD, PAD |
| 计算分子量 | 663 aa, 74 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC025718 |
| 基因名称 | PADI4 |
| Gene ID (NCBI) | 23569 |
| RRID | AB_2878398 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UM07 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PADI4/PAD4 antibody 17373-1-AP | Download protocol |
| IF protocol for PADI4/PAD4 antibody 17373-1-AP | Download protocol |
| IHC protocol for PADI4/PAD4 antibody 17373-1-AP | Download protocol |
| IP protocol for PADI4/PAD4 antibody 17373-1-AP | Download protocol |
| WB protocol for PADI4/PAD4 antibody 17373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity A bladder-blood immune barrier constituted by suburothelial perivascular macrophages restrains uropathogen dissemination | ||
Cancer Discov An African-specific variant of TP53 reveals PADI4 as a regulator of p53-mediated tumor suppression | ||
Nat Cancer Cardiomyocyte-localized CCDC25 senses NET DNA to promote doxorubicin cardiotoxicity by activating autophagic flux | ||
Bioact Mater TLR2 inhibition attenuates NET-driven inflammation and restenosis during poly-lactic acid bioresorbable scaffold degradation. | ||
Nat Commun Elimination of a neutrophil Pad4 byproduct restores stem cell-mediated bone regeneration in hyperglycemia. | ||
Nat Commun S100A12 triggers NETosis to aggravate myocardial infarction injury via the Annexin A5-calcium axis |
