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验证数据展示

  • IF Staining of human breast cancer using CL594-66386

    IF Staining of human breast cancer using CL594-66386

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66386 (PADI2 antibody) at dilution of 1:50.

  • FC experiment of HeLa using CL594-66386

    FC experiment of HeLa using CL594-66386

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PADI2 (CL594-66386, Clone:2D8C12) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL594-66386

    IF Staining of HeLa using CL594-66386

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 PADI2 antibody (CL594-66386, Clone: 2D8C12 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated PADI2 Monoclonal antibody

PADI2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-66386

产品说明书

CloneNo. 2D8C12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P, FC (Intra)

别名

PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue, HeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66386-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66386 targets PADI2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PADI2 fusion protein Ag17612 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidyl arginine deiminase, type II
别名 PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994
计算分子量 665 aa, 75 kDa
GenBank蛋白编号BC009701
基因名称 PADI2
Gene ID (NCBI) 11240
RRIDAB_2883573
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2J8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa. This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 PADI2 antibody CL594-66386Download protocol
FC protocol for CL594 PADI2 antibody CL594-66386Download protocol
Standard Protocols
Click here to view our Standard Protocols