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PADI2 Monoclonal antibody

PADI2 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66386-1-Ig

产品说明书

CloneNo. 2D8C12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, rabbit, pig

应用

WB, IHC, IF-P, ELISA and More (1)

KIAA0994, PAD H19, PAD2, PADI2, PADI2 protein, PDI 2, PDI2, Peptidylarginine deiminase II

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, HeLa cells, MCF-7 cells, pig brain tissue, rat brain tissue, mouse brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66386-1-Ig targets PADI2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, rabbit, pig
文献引用反应性human, mouse, rat
免疫原 PADI2 fusion protein Ag17612 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidyl arginine deiminase, type II
别名 KIAA0994, PAD H19, PAD2, PADI2, PADI2 protein, PDI 2, PDI2, Peptidylarginine deiminase II
计算分子量 665 aa, 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC009701
基因名称 PADI2
Gene ID (NCBI) 11240
RRIDAB_2881762
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2J8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

实验方案

Product Specific Protocols
WB protocol for PADI2 antibody 66386-1-IgDownload protocol
IHC protocol for PADI2 antibody 66386-1-IgDownload protocol
IF protocol for PADI2 antibody 66386-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Immunother Cancer

Protein citrullination as a source of cancer neoantigens.

Authors - Hiroyuki Katayama
mouseIHC,WB

Cell Death Dis

Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination

Authors - Hyun-Jung Kim
  • KD Validated
human,mouseWB,IF

Cell Mol Life Sci

ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.

Authors - Hyun-Jung Kim
  • KD Validated
ratWB

Mol Cell Proteomics

Changes in the Oligodendrocyte Progenitor Cell Proteome with Ageing.

Authors - Alerie G de la Fuente
mouseIF

Acta Pharm Sin B

Cholinergic dysfunction-induced insufficient activation of alpha7 nicotinic acetylcholine receptor drives the development of rheumatoid arthritis through promoting protein citrullination via the SP3/PAD4 pathway

Authors - Changjun Lv
humanWB

Philos Trans R Soc Lond B Biol Sci

Small molecule activates citrullination through targeting PAD2

Authors - Xue Zhang
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