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CoraLite® Plus 488-conjugated Occludin Polyclonal antibody

Occludin Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-27260

Print datasheet

Synonyms

occludin, OCLN

验证数据展示

  • IF Staining of MCF-7 using CL488-27260

    IF Staining of MCF-7 using CL488-27260

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:800.

  • FC experiment of MCF-7 using CL488-27260

    FC experiment of MCF-7 using CL488-27260

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Occludin (CL488-27260) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inMCF-7 cells, Caco-2 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 27260-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-27260 is Occludin Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen Occludin fusion protein Ag26173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name occludin
Synonyms occludin, OCLN
Calculated Molecular Weight 522 aa, 59 kDa
GenBank Accession NumberBC029886
Gene Symbol OCLN
Gene ID (NCBI) 4950
RRIDAB_2919212
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16625
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Occludin antibody CL488-27260Download protocol
FC protocol for CL Plus 488 Occludin antibody CL488-27260Download protocol
Standard Protocols
Click here to view our Standard Protocols