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验证数据展示

  • WB analysis using CL488-66823

    WB analysis using CL488-66823

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66823 (OGT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated OGT Monoclonal antibody

OGT Monoclonal Antibody for WB
Cat No. CL488-66823

产品说明书

CloneNo. 2B2A6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB

别名

2B2A6, EC:2.4.1.255, HRNT1, O GLCNAC, O-GlcNAc transferase subunit p110

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66823-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66823 targets OGT in WB applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag28402

Product name: Recombinant human OGT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC014434

Sequence: MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQD

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
别名 2B2A6, EC:2.4.1.255, HRNT1, O GLCNAC, O-GlcNAc transferase subunit p110
计算分子量 1046 aa, 117 kDa
观测分子量 110 kDa
GenBank蛋白编号BC014434
基因名称 OGT
Gene ID (NCBI) 8473
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15294
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 OGT antibody CL488-66823Download protocol
Standard Protocols
Click here to view our Standard Protocols