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验证数据展示

  • WB analysis using 85726-1-RR

    WB analysis using 85726-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85726-1-RR (OCT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85726-1-RR

    IF Staining of HeLa using 85726-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCT1 antibody (85726-1-RR, Clone: 243074D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85726-1-RR

    Affinity and Kinetic Characterization of 85726-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85726-1-RR against Human OCT1 were performed. The affinity constant is below 1 pM.

OCT1 Recombinant antibody

OCT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85726-1-RR

产品说明书

CloneNo. 243074D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, NCCIT cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85726-1-RR targets OCT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 OCT1 fusion protein Ag0586 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 POU class 2 homeobox 1
别名 NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1
计算分子量 76 kDa
观测分子量 90 kDa
GenBank蛋白编号BC003571
基因名称 POU2F1
Gene ID (NCBI) 5451
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14859
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].

实验方案

Product Specific Protocols
WB protocol for OCT1 antibody 85726-1-RRDownload protocol
IF protocol for OCT1 antibody 85726-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols