• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85726-1-RR (same clone as 85726-1-PBS)

    WB analysis using 85726-1-RR (same clone as 85726-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85726-1-RR (OCT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85726-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85726-1-RR (same clone as 85726-1-PBS)

    IF Staining of HeLa using 85726-1-RR (same clone as 85726-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCT1 antibody (85726-1-RR, Clone: 243074D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85726-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85726-1-RR

    Affinity and Kinetic Characterization of 85726-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85726-1-RR against Human OCT1 were performed. The affinity constant is below 1 pM.

OCT1 Recombinant antibody, PBS Only

OCT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85726-1-PBS

产品说明书

CloneNo. 243074D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

85726-1-PBS targets OCT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 OCT1 fusion protein Ag0586 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 POU class 2 homeobox 1
别名 NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1
计算分子量 76 kDa
观测分子量 90 kDa
GenBank蛋白编号BC003571
基因名称 POU2F1
Gene ID (NCBI) 5451
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14859
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].