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验证数据展示

  • WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

    WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

  • WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

    WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

  • WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

    WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

    Mouse brain tissue was subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02088-1

    Cytometric bead array standard curve of MP02088-1

    Cytometric bead array standard curve of MP02088-1, MOUSE Nov/CCN3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85731-2-PBS. Detection antibody: 85731-1-PBS. Standard: Eg2856. Range: 1.563-200 ng/mL.

Mouse NOV/CCN3 Recombinant antibody, PBS Only (Detector)

NOV/CCN3 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 85731-1-PBS

产品说明书

CloneNo. 250054B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85731-1-PBS targets NOV/CCN3 as part of a matched antibody pair:

MP02088-1: 85731-2-PBS capture and 85731-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NOV/CCN3 fusion protein Eg2856 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nephroblastoma overexpressed gene
别名 CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov
计算分子量39 kDa
观测分子量 46 kDa
GenBank蛋白编号NM_010930.4
基因名称 Nov
Gene ID (NCBI) 18133
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ64299
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCN family member 3 (CCN3), also known as Nephroblastoma overexpressed (NOV) protein, is a member of the CCN protein family (PMID: 1309586). CCN3 is predominantly expressed in the cytoplasm, nucleus, and extracellular space. As a subset of extracellular matrix proteins, it can directly bind to the integrin receptor family on the cell surface and participate in signal transduction between the cell surface and the extracellular matrix structure (PMID: 37378812). CCN3 exhibits anti-proliferative activity in normal cells and most tumor cells (PMID: 17340618). CCN3 co-localizes with connexin 43 (C×43) in the plasma membrane, and these two proteins physically interact to inhibit cell growth (PMID: 15213231). CCN3 can induce myofibroblast differentiation through the Notch pathway (PMID: 12050162). CCN can promote adhesion of endothelial cells, vascular smooth muscle cells, and fibroblasts through heparan sulfate proteoglycans (HSPG) (PMID: 30158025).