验证数据展示
经过测试的应用
| Positive WB detected in | A172 cells, HEK-293T cells, mouse brain tissue, HeLa cells, mouse thymus tissue, Caco-2 cells, rat thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85731-1-RR targets NOV/CCN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | NOV/CCN3 fusion protein Eg2856 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nephroblastoma overexpressed gene |
| 别名 | CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov |
| 计算分子量 | 39 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | NM_010930.4 |
| 基因名称 | Nov |
| Gene ID (NCBI) | 18133 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q64299 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCN family member 3 (CCN3), also known as Nephroblastoma overexpressed (NOV) protein, is a member of the CCN protein family (PMID: 1309586). CCN3 is predominantly expressed in the cytoplasm, nucleus, and extracellular space. As a subset of extracellular matrix proteins, it can directly bind to the integrin receptor family on the cell surface and participate in signal transduction between the cell surface and the extracellular matrix structure (PMID: 37378812). CCN3 exhibits anti-proliferative activity in normal cells and most tumor cells (PMID: 17340618). CCN3 co-localizes with connexin 43 (C×43) in the plasma membrane, and these two proteins physically interact to inhibit cell growth (PMID: 15213231). CCN3 can induce myofibroblast differentiation through the Notch pathway (PMID: 12050162). CCN can promote adhesion of endothelial cells, vascular smooth muscle cells, and fibroblasts through heparan sulfate proteoglycans (HSPG) (PMID: 30158025).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOV/CCN3 antibody 85731-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |