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验证数据展示

  • WB analysis using 85622-2-RR (same clone as 85622-2-PBS)

    WB analysis using 85622-2-RR (same clone as 85622-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85622-2-RR (Ngfr antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85622-2-PBS in a different storage buffer formulation.

  • WB analysis using 85622-2-RR (same clone as 85622-2-PBS)

    WB analysis using 85622-2-RR (same clone as 85622-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85622-2-RR (Ngfr antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85622-2-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 85622-2-RR (same clone as 85622-2-PBS)

    IF Staining of PC-12 using 85622-2-RR (same clone as 85622-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using NGFR/TNFRSF16 antibody (85622-2-RR, Clone: 242634F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85622-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02004-1

    Cytometric bead array standard curve of MP02004-1

    Cytometric bead array standard curve of MP02004-1, MOUSE NGFR/TNFRSF16 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85622-3-PBS. Detection antibody: 85622-2-PBS. Standard: Eg2651. Range: 0.781-50 ng/mL.

  • Cytometric bead array standard curve of MP02004-2

    Cytometric bead array standard curve of MP02004-2

    Cytometric bead array standard curve of MP02004-2, MOUSE NGFR/TNFRSF16 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85622-2-PBS. Detection antibody: 85622-1-PBS. Standard: Eg2651. Range: 0.781-100 ng/mL.

Mouse NGFR/TNFRSF16 Recombinant antibody, PBS Only (Capture/Detector)

NGFR/TNFRSF16 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85622-2-PBS

产品说明书

CloneNo. 242634F10

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85622-2-PBS targets NGFR/TNFRSF16 as part of a matched antibody pair:

MP02004-1: 85622-3-PBS capture and 85622-2-PBS detection (validated in Cytometric bead array)

MP02004-2: 85622-2-PBS capture and 85622-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 NGFR/TNFRSF16 fusion protein Eg2651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nerve growth factor receptor (TNFR superfamily, member 16)
别名 CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR
计算分子量46 kDa
观测分子量70-75 kDa
GenBank蛋白编号NM_033217.3
基因名称 Ngfr
Gene ID (NCBI) 18053
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Z0W1-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

p75NTR, also named TNFRSF16, Gp80-LNGFR, p75 ICD, and CD271, is a low-affinity receptor that can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as the cell death of neural cells. The antibody is specific to NGFR.