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验证数据展示

  • WB analysis using 85622-2-RR

    WB analysis using 85622-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85622-2-RR (Ngfr antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85622-2-RR

    WB analysis using 85622-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85622-2-RR (Ngfr antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-12 using 85622-2-RR

    IF Staining of PC-12 using 85622-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using NGFR/TNFRSF16 antibody (85622-2-RR, Clone: 242634F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NGFR/TNFRSF16 Recombinant monoclonal antibody

NGFR/TNFRSF16 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85622-2-RR

产品说明书

CloneNo. 242634F10

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, ELISA

别名

CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, rat uterus tissue, mouse uterus tissue
Positive IF/ICC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85622-2-RR targets NGFR/TNFRSF16 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2651

Product name: Recombinant mouse Ngfr protein (C-rFc)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 32-252 aa of NM_033217.3

Sequence: KETCSTGMYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECLGLQSMSAPCVEADDAVCRCSYGYYQDEETGRCEACSVCGVGSGLVFSCQDKQNTVCEECPEGTYSDEANHVDPCLPCTVCEDTERQLRECTPWADAECEEIPGRWITRSTPPEGSDVTTPSTQEPEAPPERDLIASTVADTVTTVMGSSQPVVTRGTAD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nerve growth factor receptor (TNFR superfamily, member 16)
别名 CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR
计算分子量46 kDa
观测分子量70-75 kDa
GenBank蛋白编号NM_033217.3
基因名称 Ngfr
Gene ID (NCBI) 18053
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Z0W1-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p75NTR, also named TNFRSF16, Gp80-LNGFR, p75 ICD, and CD271, is a low-affinity receptor that can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as the cell death of neural cells. The antibody is specific to NGFR.

实验方案

Product Specific Protocols
IF protocol for NGFR/TNFRSF16 antibody 85622-2-RRDownload protocol
WB protocol for NGFR/TNFRSF16 antibody 85622-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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