CoraLite® Plus 647-conjugated TGF beta 1 Monoclonal antibody
TGF beta 1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1E3E9
验证数据展示
经过测试的应用
Positive FC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 69012-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-69012 targets TGF beta 1 in FC (Intra) applications and shows reactivity with Human samples.
Tested Applications | FC (Intra) |
Tested Reactivity | Human |
Immunogen | TGF beta 1 fusion protein HZ-1011 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | transforming growth factor, beta 1 |
Synonyms | CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta, TGF-beta1 |
Gene Symbol | TGFB1 |
Gene ID (NCBI) | 7040 |
RRID | AB_2920324 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).
This antibody is used to Block/Neutralize TGF beta 1 protein.
This antibody is used to Block/Neutralize TGF beta 1 protein.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 TGF beta 1 antibody CL647-69012 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |