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验证数据展示

  • IF Staining of MCF-7 using CL488-83223-4

    IF Staining of MCF-7 using CL488-83223-4

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Nectin-4/PVRL4 antibody (CL488-83223-4, Clone: 240009F2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Nectin-4/PVRL4 Recombinant monoclonal antibody

Nectin-4/PVRL4 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-83223-4

产品说明书

CloneNo. 240009F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83223-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83223-4 targets Nectin-4/PVRL4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Eg0415

Product name: Recombinant human nectin 4 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 32-349 aa of BC010423

Sequence: GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poliovirus receptor-related 4
别名 Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR
计算分子量 510 aa, 55 kDa
GenBank蛋白编号BC010423
基因名称 Nectin-4
Gene ID (NCBI) 81607
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96NY8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Nectin-4/PVRL4 antibody CL488-83223-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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