• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    FC experiment of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    1x10^6 MCF-7 cells were surface stained with 0.25 ug Nectin-4/PVRL4 Recombinant Antibody (83223-4-RR, Clone: 240009F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    IF Staining of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (83223-4-RR, Clone: 240009F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83223-4-RR

    Affinity and Kinetic Characterization of 83223-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83223-4-RR against Human Nectin-4/PVRL4 were performed. The affinity constant is below 1 pM.

Nectin-4/PVRL4 Recombinant monoclonal antibody, PBS Only

Nectin-4/PVRL4 Uni-rAb® Recombinant Antibody for IF/ICC, FC, Indirect ELISA
Cat No. 83223-4-PBS

产品说明书

CloneNo. 240009F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC, Indirect ELISA

别名

Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

产品信息

83223-4-PBS targets Nectin-4/PVRL4 in IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0415

Product name: Recombinant human nectin 4 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 32-349 aa of BC010423

Sequence: GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=2.28 x 10-10M
KOff=2.98 x 10-5M
KOn=2.62 x 105M
全称 poliovirus receptor-related 4
别名 Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR
计算分子量 510 aa, 55 kDa
GenBank蛋白编号BC010423
基因名称 Nectin-4
Gene ID (NCBI) 81607
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96NY8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

||
New chat Able
正在加载，请稍候...