验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, K-562 cells, human kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86052-1-RR targets Napsin A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Napsin A fusion protein Ag9721 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | napsin A aspartic peptidase |
| 别名 | NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.- |
| 计算分子量 | 420 aa, 45 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC017842 |
| 基因名称 | Napsin A |
| Gene ID (NCBI) | 9476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O96009 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Napsin A antibody 86052-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |