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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

    WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

    WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

    IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 85939-4-RR (same clone as 85939-4-PBS)

    IP experiment of HeLa using 85939-4-RR (same clone as 85939-4-PBS)

    IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

    IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85939-4-RR

    Affinity and Kinetic Characterization of 85939-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

NUMB Recombinant antibody, PBS Only

NUMB Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85939-4-PBS

产品说明书

CloneNo. 250417F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

C14orf41, h Numb, h-Numb

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85939-4-PBS targets NUMB in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUMB fusion protein Ag31553 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 numb homolog (Drosophila)
别名 C14orf41, h Numb, h-Numb
计算分子量71KD
观测分子量66-71 kDa
GenBank蛋白编号NM_001005743
基因名称 NUMB
Gene ID (NCBI) 8650
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49757
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.