验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, A-204 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SKOV-3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
85939-4-RR targets NUMB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | NUMB fusion protein Ag31553 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | numb homolog (Drosophila) |
别名 | C14orf41, h Numb, h-Numb |
计算分子量 | 71KD |
观测分子量 | 66-71 kDa |
GenBank蛋白编号 | NM_001005743 |
基因名称 | NUMB |
Gene ID (NCBI) | 8650 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P49757 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.
实验方案
Product Specific Protocols | |
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WB protocol for NUMB antibody 85939-4-RR | Download protocol |
IHC protocol for NUMB antibody 85939-4-RR | Download protocol |
IP protocol for NUMB antibody 85939-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |