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验证数据展示

  • WB analysis using 85939-4-RR

    WB analysis using 85939-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85939-4-RR

    WB analysis using 85939-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 85939-4-RR

    IHC staining of human breast cancer using 85939-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 85939-4-RR

    IP experiment of HeLa using 85939-4-RR

    IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug.

  • IHC staining of human breast cancer using 85939-4-RR

    IHC staining of human breast cancer using 85939-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85939-4-RR

    Affinity and Kinetic Characterization of 85939-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

NUMB Recombinant antibody

NUMB Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85939-4-RR

产品说明书

CloneNo. 250417F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

C14orf41, h Numb, h-Numb

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, A-204 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85939-4-RR targets NUMB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUMB fusion protein Ag31553 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 numb homolog (Drosophila)
别名 C14orf41, h Numb, h-Numb
计算分子量71KD
观测分子量66-71 kDa
GenBank蛋白编号NM_001005743
基因名称 NUMB
Gene ID (NCBI) 8650
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.

实验方案

Product Specific Protocols
WB protocol for NUMB antibody 85939-4-RRDownload protocol
IHC protocol for NUMB antibody 85939-4-RRDownload protocol
IP protocol for NUMB antibody 85939-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols