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验证数据展示

  • WB analysis using 85939-4-RR

    WB analysis using 85939-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85939-4-RR

    WB analysis using 85939-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 85939-4-RR

    IHC staining of human breast cancer using 85939-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 85939-4-RR

    IP experiment of HeLa using 85939-4-RR

    IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug.

  • IHC staining of human breast cancer using 85939-4-RR

    IHC staining of human breast cancer using 85939-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85939-4-RR

    Affinity and Kinetic Characterization of 85939-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

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NUMB Recombinant monoclonal antibody

NUMB Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85939-4-RR

产品说明书

CloneNo. 250417F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

C14orf41, h Numb, h-Numb

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, A-204 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85939-4-RR targets NUMB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31553

Product name: Recombinant human NUMB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 510-651 aa of NM_001005743

Sequence: SYPVANGMPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNPFSSDLQKTFEIEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 numb homolog (Drosophila)
别名 C14orf41, h Numb, h-Numb
计算分子量71KD
观测分子量66-71 kDa
GenBank蛋白编号NM_001005743
基因名称 NUMB
Gene ID (NCBI) 8650
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.

实验方案

Product Specific Protocols
WB protocol for NUMB antibody 85939-4-RRDownload protocol
IHC protocol for NUMB antibody 85939-4-RRDownload protocol
IP protocol for NUMB antibody 85939-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols