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验证数据展示

  • WB analysis using 84074-4-RR

    WB analysis using 84074-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84074-4-RR

    WB analysis using 84074-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84074-4-RR

    Affinity and Kinetic Characterization of 84074-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84074-4-RR against Human NOTCH2 were performed. The affinity constant is 1.86 nM.

NOTCH2 Recombinant antibody

NOTCH2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84074-4-RR

产品说明书

CloneNo. 241162A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-87 MG cells, HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NOTCH2 Ready-To-Use IHC Kit. NOTCH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84074-4-RR targets NOTCH2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 NOTCH2 fusion protein Ag29413 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Notch homolog 2 (Drosophila)
别名 Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2
计算分子量 265 kDa
观测分子量 110 kDa, 265kDa
GenBank蛋白编号NM_024408
基因名称 NOTCH2
Gene ID (NCBI) 4853
RRIDAB_3671644
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ04721
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).

实验方案

Product Specific Protocols
WB protocol for NOTCH2 antibody 84074-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols