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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

  • WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84074-4-RR

    Affinity and Kinetic Characterization of 84074-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84074-4-RR against Human NOTCH2 were performed. The affinity constant is 1.86 nM.

NOTCH2 Recombinant antibody, PBS Only

NOTCH2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84074-4-PBS

产品说明书

CloneNo. 241162A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NOTCH2 Ready-To-Use IHC Kit. NOTCH2 primary antibody included.

产品信息

84074-4-PBS targets NOTCH2 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 NOTCH2 fusion protein Ag29413 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Notch homolog 2 (Drosophila)
别名 Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2
计算分子量 265 kDa
观测分子量 110 kDa, 265kDa
GenBank蛋白编号NM_024408
基因名称 NOTCH2
Gene ID (NCBI) 4853
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ04721
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).