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验证数据展示

  • IF Staining of U2OS using CL488-28509

    IF Staining of U2OS using CL488-28509

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 NOC2L antibody (CL488-28509) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of U2OS using CL488-28509

    FC experiment of U2OS using CL488-28509

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated NOC2L Polyclonal antibody (CL488-28509)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated NOC2L Polyclonal antibody

NOC2L Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28509

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

Protein NOC2 homolog, Nucleolar complex protein 2 homolog, Novel INHAT repressor, NOC2-like protein, NIR

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy® NOC2L Ready-To-Use IHC Kit. NOC2L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28509-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28509 targets NOC2L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26952

Product name: Recombinant human NOC2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC003555

Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar complex associated 2 homolog (S. cerevisiae)
别名 Protein NOC2 homolog, Nucleolar complex protein 2 homolog, Novel INHAT repressor, NOC2-like protein, NIR
计算分子量 749 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC003555
基因名称 NOC2L
Gene ID (NCBI) 26155
RRIDAB_3672828
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3T9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NOC2L antibody CL488-28509Download protocol
IF protocol for CL Plus 488 NOC2L antibody CL488-28509Download protocol
Standard Protocols
Click here to view our Standard Protocols
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