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NOC2L Polyclonal antibody

NOC2L Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28509-1-AP

Print datasheet

Synonyms

DKFZp564C186, FLJ35172, NIR, NOC2 like protein, NOC2L, Protein NOC2 homolog

验证数据展示

  • WB analysis using 28509-1-AP

    WB analysis using 28509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28509-1-AP (NOC2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28509-1-AP

    IHC staining of human prostate cancer using 28509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28509-1-AP

    IHC staining of human prostate cancer using 28509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 28509-1-AP

    IF Staining of U2OS using 28509-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 28509-1-AP (NOC2L antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin (red).

经过测试的应用

Positive WB detected inU2OS cells, HEK-293T cells, HuH-7 cells, mouse cerebellum tissue, mouse stomach tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28509-1-AP targets NOC2L in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen NOC2L fusion protein Ag26952 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleolar complex associated 2 homolog (S. cerevisiae)
Synonyms DKFZp564C186, FLJ35172, NIR, NOC2 like protein, NOC2L, Protein NOC2 homolog
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC003555
Gene Symbol NOC2L
Gene ID (NCBI) 26155
RRIDAB_2881160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3T9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

实验方案

Product Specific Protocols
WB protocol for NOC2L antibody 28509-1-APDownload protocol
IHC protocol for NOC2L antibody 28509-1-APDownload protocol
IF protocol for NOC2L antibody 28509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols