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验证数据展示

  • WB analysis using 28509-1-AP

    WB analysis using 28509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28509-1-AP (NOC2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28509-1-AP

    IHC staining of human prostate cancer using 28509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28509-1-AP

    IHC staining of human prostate cancer using 28509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 28509-1-AP

    IF Staining of U2OS using 28509-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 28509-1-AP (NOC2L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin (red).

NOC2L Polyclonal antibody

NOC2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28509-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp564C186, FLJ35172, NIR, NOC2 like protein, NOC2L, Protein NOC2 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, HEK-293T cells, HuH-7 cells, mouse cerebellum tissue, mouse stomach tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28509-1-AP targets NOC2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag26952

Product name: Recombinant human NOC2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC003555

Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar complex associated 2 homolog (S. cerevisiae)
别名 DKFZp564C186, FLJ35172, NIR, NOC2 like protein, NOC2L, Protein NOC2 homolog
计算分子量 749 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC003555
基因名称 NOC2L
Gene ID (NCBI) 26155
RRIDAB_2881160
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3T9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

实验方案

Product Specific Protocols
WB protocol for NOC2L antibody 28509-1-APDownload protocol
IHC protocol for NOC2L antibody 28509-1-APDownload protocol
IF protocol for NOC2L antibody 28509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols