验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy NME1 Ready-To-Use IHC Kit. NME1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85038-7-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85038-7 targets NME1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | NME1 fusion protein Ag1548 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | non-metastatic cells 1, protein (NM23A) expressed in |
| 别名 | 242670D5, AWD, EC:2.7.4.6, GAAD, Granzyme A activated DNase |
| 计算分子量 | 17 kDa |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | BC018994 |
| 基因名称 | NME1 |
| Gene ID (NCBI) | 4830 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15531 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NME1 antibody CL488-85038-7 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |