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验证数据展示

  • WB analysis using 85038-7-RR (same clone as 85038-7-PBS)

    WB analysis using 85038-7-RR (same clone as 85038-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85038-7-RR (NME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 85038-7-RR (same clone as 85038-7-PBS)

    FC experiment of HepG2 using 85038-7-RR (same clone as 85038-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NME1 Recombinant antibody (85038-7-RR, Clone:242670D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 85038-7-RR (same clone as 85038-7-PBS)

    IF Staining of MCF-7 using 85038-7-RR (same clone as 85038-7-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NME1 antibody (85038-7-RR, Clone: 242670D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

NME1 Recombinant monoclonal antibody, PBS Only

NME1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85038-7-PBS

产品说明书

CloneNo. 242670D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

242670D5, AWD, EC:2.7.4.6, GAAD, Granzyme A activated DNase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NME1 Ready-To-Use IHC Kit. NME1 primary antibody included.

产品信息

85038-7-PBS targets NME1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1548

Product name: Recombinant human NME1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC018994

Sequence: MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 non-metastatic cells 1, protein (NM23A) expressed in
别名 242670D5, AWD, EC:2.7.4.6, GAAD, Granzyme A activated DNase
计算分子量 17 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC018994
基因名称 NME1
Gene ID (NCBI) 4830
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15531
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination. It has 3 isoforms produced by alternative splicing. Refer to the epitope and protein detection, this antibody specifically recognizes NME1.

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