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验证数据展示

  • WB analysis of mouse heart using 83131-8-RR

    WB analysis of mouse heart using 83131-8-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83131-8-RR (NLRP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83131-8-RR

    FC experiment of A431 using 83131-8-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83131-8-RR

    Affinity and Kinetic Characterization of 83131-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83131-8-RR against Human NLRP10 were performed. The affinity constant is 0.899 nM.

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NLRP10 Recombinant monoclonal antibody

NLRP10 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83131-8-RR

产品说明书

CloneNo. 230402C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

230402C1, CLR11.1, NACHT, LRR and PYD domains-containing protein 10, NALP10, NOD8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83131-8-RR targets NLRP10 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26402

Product name: Recombinant human NLRP10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC104957

Sequence: MAMAKARKPREALLWALSDLEENDFKKLKFYLRDMTLSEGQPPLARGELEGLIPVDLAELLISKYGEKEAVKVVLKGLKVMNLLELVDQLSHICLHDYREVYREHVRCLEEWQEAGVNGRYNQVLLVAKPSSESPESLACPFPEQELESVTVEALFDSGEKPSLAPSLVVLQGSAGTGKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NLR family, pyrin domain containing 10
别名 230402C1, CLR11.1, NACHT, LRR and PYD domains-containing protein 10, NALP10, NOD8
计算分子量 655 aa, 75 kDa
观测分子量75 kDa
GenBank蛋白编号BC104957
基因名称 NLRP10
Gene ID (NCBI) 338322
RRIDAB_3670834
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ86W26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLRP10 is a member of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). NLRP10 likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.

实验方案

Product Specific Protocols
WB protocol for NLRP10 antibody 83131-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols