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验证数据展示

  • WB analysis of mouse heart using 83131-8-RR

    WB analysis of mouse heart using 83131-8-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83131-8-RR (NLRP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83131-8-RR

    FC experiment of A431 using 83131-8-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83131-8-RR

    Affinity and Kinetic Characterization of 83131-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83131-8-RR against Human NLRP10 were performed. The affinity constant is 0.899 nM.

NLRP10 Recombinant antibody

NLRP10 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83131-8-RR

产品说明书

CloneNo. 230402C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Nucleotide-binding oligomerization domain protein 8, NOD8, NALP10, NACHT, LRR and PYD domains-containing protein 10, CLR11.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive FC (Intra) detected inA431 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83131-8-RR targets NLRP10 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 NLRP10 fusion protein Ag26402 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NLR family, pyrin domain containing 10
别名 Nucleotide-binding oligomerization domain protein 8, NOD8, NALP10, NACHT, LRR and PYD domains-containing protein 10, CLR11.1
计算分子量 655 aa, 75 kDa
观测分子量75 kDa
GenBank蛋白编号BC104957
基因名称 NLRP10
Gene ID (NCBI) 338322
RRIDAB_3670834
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86W26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLRP10 is a member of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). NLRP10 likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.

实验方案

Product Specific Protocols
WB protocol for NLRP10 antibody 83131-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols