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CoraLite® Plus 488-conjugated NIT2 Monoclonal antibody

NIT2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E5B1

Cat No : CL488-68169

Print datasheet

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

验证数据展示

  • IF Staining of U-251 using CL488-68169

    IF Staining of U-251 using CL488-68169

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 NIT2 antibody (CL488-68169, Clone: 1E5B1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 68169-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68169 targets NIT2 in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat, pig
Immunogen NIT2 fusion protein Ag9889 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nitrilase family, member 2
Synonyms NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2
Calculated Molecular Weight 276 aa, 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC020620
Gene Symbol NIT2
Gene ID (NCBI) 56954
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQR4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NIT2 antibody CL488-68169Download protocol
Standard Protocols
Click here to view our Standard Protocols