验证数据展示
经过测试的应用
| Positive FC detected in | SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-98896-5 targets NGFR/TNFRSF16 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg4829 Product name: Recombinant Human NGFR/TNFRSF16 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 29-250 aa of NM_002507.4 Sequence: KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nerve growth factor receptor (TNFR superfamily, member 16) |
| 别名 | NGFR, CD271, Gp80 LNGFR, Gp80-LNGFR, Low-affinity nerve growth factor receptor |
| 计算分子量 | 45 kDa |
| GenBank蛋白编号 | NM_002507.4 |
| 基因名称 | p75NTR |
| Gene ID (NCBI) | 4804 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08138-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The nerve growth factor (NGF) receptor (NGFR), also known as p75NTR, TNFRSF16, belongs to the tumor necrosis factor receptor superfamily, which is a low-affinity receptor of neurotrophins including NGF, brain-derived neurotrophic factor (BDNF), and neurotrophin-3, -4, and -5. It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells (PMID: 38307025, 16424897).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 NGFR/TNFRSF16 antibody CL488-98896-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
