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验证数据展示

  • FC experiment of SK-N-SH using 98896-5-RR

    FC experiment of SK-N-SH using 98896-5-RR

    1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human NGFR/TNFRSF16 Rabbit RecAb (98896-5-RR, Clone: 260065A1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected CHO-K1 using 98896-5-RR

    FC experiment of Transfected CHO-K1 using 98896-5-RR

    1x10^6 CHO-K1 (left) or NGFR/TNFRSF16 transfected CHO-K1 (right) or were surface stained with 0.25 ug Anti-Human NGFR/TNFRSF16 Rabbit RecAb (98896-5-RR, Clone: 260065A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected CHO-K1 using 98896-5-RR

    FC experiment of Transfected CHO-K1 using 98896-5-RR

    1x10^6 CHO-K1 (left) or NGFR/TNFRSF16 transfected CHO-K1 (right) were surface stained with 0.25 ug Anti-Human NGFR/TNFRSF16 Rabbit RecAb (98896-5-RR, Clone: 260065A1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Anti-Human NGFR/TNFRSF16 Rabbit Recombinant Antibody

NGFR/TNFRSF16 Uni-rAb® Recombinant Antibody for FC
Cat No. 98896-5-RR

产品说明书

CloneNo. 260065A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

NGFR, CD271, Gp80 LNGFR, Gp80-LNGFR, Low-affinity nerve growth factor receptor

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inSK-N-SH cells, Transfected CHO-K1 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98896-5-RR targets NGFR/TNFRSF16 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg4829

Product name: Recombinant Human NGFR/TNFRSF16 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-250 aa of NM_002507.4

Sequence: KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nerve growth factor receptor (TNFR superfamily, member 16)
别名 NGFR, CD271, Gp80 LNGFR, Gp80-LNGFR, Low-affinity nerve growth factor receptor
计算分子量45 kDa
GenBank蛋白编号NM_002507.4
基因名称 p75NTR
Gene ID (NCBI) 4804
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08138-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The nerve growth factor (NGF) receptor (NGFR), also known as p75NTR, TNFRSF16, belongs to the tumor necrosis factor receptor superfamily, which is a low-affinity receptor of neurotrophins including NGF, brain-derived neurotrophic factor (BDNF), and neurotrophin-3, -4, and -5. It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells (PMID: 38307025, 16424897).

实验方案

Product Specific Protocols
FC protocol for NGFR/TNFRSF16 antibody 98896-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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