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验证数据展示

  • WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve of MP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

  • IF Staining of SKOV-3 using 66924-1-Ig (same clone as 66924-1-PBS)

    IF Staining of SKOV-3 using 66924-1-Ig (same clone as 66924-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using NFATC3 antibody (66924-1-Ig, Clone: 1C1H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

  • IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

    IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFATC3 antibody included
  • IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

    IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFATC3 antibody included

NFATC3 Monoclonal antibody, PBS Only (Detector)

NFATC3 Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 66924-1-PBS

产品说明书

CloneNo. 1C1H8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

1C1H8, NF AT4, NF ATc3, NFAT4, NF-AT4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.

产品信息

66924-1-PBS targets NFATC3 as part of a matched antibody pair:

MP50022-1: 66924-2-PBS capture and 66924-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27306

Product name: Recombinant human NFATC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-205 aa of BC001050

Sequence: MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
别名 1C1H8, NF AT4, NF ATc3, NFAT4, NF-AT4
计算分子量 116 kDa
观测分子量 160 kDa
GenBank蛋白编号BC001050
基因名称 NFATC3
Gene ID (NCBI) 4775
RRIDAB_2882251
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ12968
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

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