NFATC3 Monoclonal antibody
NFATC3 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C1H8
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, Daudi cells |
Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66924-1-Ig targets NFATC3 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human |
| Immunogen | NFATC3 fusion protein Ag27306 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 |
| Synonyms | NF AT4, NF ATc3, NFAT4, NFATC3, NFATX |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC001050 |
| Gene Symbol | NFATC3 |
| Gene ID (NCBI) | 4775 |
| RRID | AB_2882251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12968 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFATC3 antibody 66924-1-Ig | Download protocol |
| IHC protocol for NFATC3 antibody 66924-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
