NEU2 Recombinant antibody

NEU2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83934-1-RR

产品说明书

CloneNo. 240685B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

240685B3, Cytosolic sialidase, EC:3.2.1.18, N acetyl alpha neuraminidase 2, N-acetyl-alpha-neuraminidase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83934-1-RR targets NEU2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NEU2 fusion protein Ag17386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sialidase 2 (cytosolic sialidase)
别名 240685B3, Cytosolic sialidase, EC:3.2.1.18, N acetyl alpha neuraminidase 2, N-acetyl-alpha-neuraminidase 2
计算分子量 380 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC107053
基因名称 NEU2
Gene ID (NCBI) 4759
RRIDAB_3671515
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y3R4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)

实验方案

Product Specific Protocols
WB protocol for NEU2 antibody 83934-1-RRDownload protocol
IHC protocol for NEU2 antibody 83934-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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